On 18 -19 August 2015 the BASTION Project held practical workshops dedicated to high-performance automated DNA isolation using the platform Chemagen MSM1 (Perkin Elmer) and an average productive method of analysis of genotypes using the platform FlexMap 3D (Luminex) and biorobot Janus (Perkin Elmer). The practical workshops were conducted at the Chair and Department of Internal Medicine, Hypertension and Angiology at the Medical University of Warsaw.
During the workshop, participants had the opportunity to familiarize themselves with the process of high-performance DNA isolation using fully automated stations isolating nucleic acids Chemagen MSM1 (Perkin Elmer). The platform due to the presence of interchangeable electromagnetic heads allows the simultaneous isolation of 12 to 96 samples in a volume of 10 uL to 10 ml, based on the method of binding DNA to a polyvinyl alcohol coated on magnetic beads.
In the second part of the workshop, participants learned about the principles of operation and possibilities of 3D FlexMap Luminex platform, which combines the principle of operation of the flow cytometer recognizing many types of microspheres with the ability to determine the amount of analyte on the microsphere. During the workshop design experience using a camera has been discussed in detail on the example of genotyping.
In the practical part of the workshop, participants conducted genotyping of the three polymorphisms for 96 samples by Direct Hybridization method, in the reaction of 3-plex. The workshop ended with a seminar, during which the evaluation of genotyping method clustering results was performed.
The workshops were organized by a team from the Laboratory of the Department of Internal Medicine, Hypertension and Angiology, Medical University of Warsaw. Participants of the workshop are the employees of the Institute of Hematology and Blood Transfusion, Institute of Cardiology at the Medical University of Warsaw.